Vesna M

نویسندگان

  • VESNA M. VASIĆ
  • MIRJANA B. ČOLOVIĆ
  • DANIJELA Z. KRSTIĆ
چکیده

The aim of the study was to give an overview of the mechanism of inhibition of Na/K–ATPase and Mg–ATPase activity, the enzymes playing a key role in the active transport of monovalent cations (Na and K) across the cell membrane, induced by the ions of some transition (Cu, Zn, Fe, Co) and heavy (Hg i Cd) metals, ammonium decavanadate and noble metals complexes ([PtCl2DMSO2], [AuCl4], [PdCl4], [PdCl(dien)], [PdCl(Me4dien)]). The extensive kinetic analysis was done in order to determine kinetic parameters and the mode of interaction of Na/K–ATPase and Mg–ATPase and the investigated compounds. In addition, the ability of sulphur-donor ligands (L-cysteine and glutathione), as well as EDTA, to prevent metal ions and complexes induced inhibition of Na/K–ATPase activity and to recover enzymatic activity was investigated. Finally, development of highly sensitive and selective analytical tools on the basis of the immobilized enzyme is discussed in this paper.

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تاریخ انتشار 2009